Vehicles for Small Interfering RNA transfection: Exosomes versus Synthetic Nanocarriers
نویسندگان
چکیده
منابع مشابه
Optimal Electroporation Condition for Small Interfering RNA Transfection into MDA-MB-468 Cell Line
Background: Electroporation is a valuable tool for small interfering RNA (siRNA) delivery into cells because it efficiently transforms a wide variety of cell types. Since electroporation condition for each cell type must be determined experimentally, this study presents an optimal electroporation strategy to reproducibly and efficiently transfect MDA-MB 468 human breast cancer cell with siRNA. ...
متن کاملSmall interfering RNA; principles, applications and challenges--
Gene silencing using RNAi (RNA interference), has recently been used as a successful laboratory technique in determining the function and control of gene expression and provides a wide range of applications in molecular biology and gene therapy. RNAi is a method of suppressing gene expression. In this direction, a single-stranded RNA molecule of about 21–23 nucleotides, called siRNA (small inte...
متن کاملoptimal electroporation condition for small interfering rna transfection into mda-mb-468 cell line
background: electroporation is a valuable tool for small interfering rna (sirna) delivery into cells because it efficiently transforms a wide variety of cell types. since electroporation condition for each cell type must be determined experimentally, this study presents an optimal electroporation strategy to reproducibly and efficiently transfect mda-mb 468 human breast cancer cell with sirna. ...
متن کاملOptimal Electroporation Condition for Small Interfering RNA Transfection into MDA-MB-468 Cell Line
BACKGROUND Electroporation is a valuable tool for small interfering RNA (siRNA) delivery into cells because it efficiently transforms a wide variety of cell types. Since electroporation condition for each cell type must be determined experimentally, this study presents an optimal electroporation strategy to reproducibly and efficiently transfect MDA-MB 468 human breast cancer cell with siRNA. ...
متن کاملComparison of small interfering RNA (siRNA) delivery into bovine monocyte-derived macrophages by transfection and electroporation
The manipulation of the RNA interference pathway using small interfering RNA (siRNA) has become the most frequently used gene silencing method. However, siRNA delivery into primary cells, especially primary macrophages, is often considered challenging. Here we report the investigation of the suitability of two methodologies: transient transfection and electroporation, to deliver siRNA targeted ...
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ژورنال
عنوان ژورنال: DNA and RNA Nanotechnology
سال: 2013
ISSN: 2353-1770
DOI: 10.2478/rnan-2013-0002